Peptide synthesis is rather a technical topic that most people do not fully comprehend. If you are a research scientist who will be dealing a lot with peptides for studies, then it is likely that you will need peptide synthesis services for most of your applications.
Here is a brief look at some of the technical synthesis questions you are likely to encounter regarding the process:
What are the typical contaminants that may be experienced in peptide synthesis?
In regards to purified peptides, the contaminants you are likely to encounter are truncations and deletion of the parent sequence. This occurs following the reduction in the efficiency of the incoming peptide due to factors such as secondary structure of the growing and the steric hindrance of the growing chain.
What is deletion and truncation in peptide synthesis?
Deletion refers to when one or more amino acids from the parent sequence is missing in any part of the sequence. A truncation is when the C-terminal of the resulting peptide is shorter than the C-terminal in the parent sequence.
What is peptide purity?
Peptide purity refers to the percentage of the peptide detected through UV absorbance of charged species or certain chemical moieties, and it is obtained through analytical methods such as MS and RP-HPLC analysis.
What typical salts are associated with peptides?
If there was no exchange of acetate, the trifluoroacetate will be the dominant ion in the peptide. If, however, you desire to have any alternative salts, then you can make requests to the synthesis company for considerations.
How is the cell permeability of a peptide increased?
The permeability of a peptide sequence, which is in the public domain such as penetratin, may be added to any sequence to increase the permeability. Such are used to enhance the transportation of peptides through the membranes, and into the cells.